Gene ID: MAB_0399c

Uniprot ID: B1MFU8
Full_Name: Helicase/nuclease RecBCD subunit RecB
Length: 1081 amino acids
Description:
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.
Sequence:
Pfam Domains
Pfam ID Pfam Name Query Mabellini
PF00580 UvrD/REP helicase N-terminal domain
PF13361 UvrD-like helicase C-terminal domain
EC Characterization
EC ID EC Name Query Mabellini
3.-.-.- Hydrolases
3.1.-.- Hydrolases - Acting on ester bonds
3.1.11.- Hydrolases - Acting on ester bonds - Exodeoxyribonucleases producing 5'-phosphomonoesters
3.1.11.5 Exodeoxyribonuclease V
Model Quality Coverage Length Model Start Model End Ligand Templates Download
55.39 34.32 371 323 693 None d1w36b2 PDB mmCIF
9.19 28.21 305 323 627 None d3hjha1 PDB mmCIF
48.72 34.32 371 323 693 None d1w36b2 PDB mmCIF
48.72 34.32 371 323 693 None d1w36b2 PDB mmCIF
48.72 34.32 371 323 693 None d1w36b2 PDB mmCIF
Model Quality Coverage Length Model Start Model End Ligand Templates Download
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