Gene ID: MAB_0398c

Uniprot ID: B1MFU7
Full_Name: Helicase/nuclease RecBCD subunit RecD
Length: 552 amino acids
Description:
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Sequence:
Pfam Domains
Pfam ID Pfam Name Query Mabellini
PF13538 UvrD-like helicase C-terminal domain
PF13604 AAA domain
EC Characterization
EC ID EC Name Query Mabellini
3.-.-.- Hydrolases
3.1.-.- Hydrolases - Acting on ester bonds
3.1.11.- Hydrolases - Acting on ester bonds - Exodeoxyribonucleases producing 5'-phosphomonoesters
3.1.11.5 Exodeoxyribonuclease V
Model Quality Coverage Length Model Start Model End Ligand Templates Download
86.2 99.28 548 1 548 None 1w36G PDB mmCIF
78.8 99.28 548 1 548 None 1w36G PDB mmCIF
26.17 11.96 66 411 476 None 3e1sA04 PDB mmCIF
63.43 17.75 98 1 98 None 1w36D01 PDB mmCIF
63.43 17.75 98 1 98 None 1w36D01 PDB mmCIF
Model Quality Coverage Length Model Start Model End Ligand Templates Download
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