Gene ID: MAB_0275

Uniprot ID: B1MFH5
Full_Name: tRNA-guanine transglycosylase
Length: 406 amino acids
Description:
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Sequence:
Pfam Domains
Pfam ID Pfam Name Query Mabellini
PF01702 Queuine tRNA-ribosyltransferase
EC Characterization
EC ID EC Name Query Mabellini
2.-.-.- Transferases
2.4.-.- Transferases - Glycosyltransferases
2.4.2.- Transferases - Glycosyltransferases - Pentosyltransferases
2.4.2.29 tRNA-guanine(34) transglycosylase
Model Quality Coverage Length Model Start Model End Ligand Templates Download
74.71 99.51 404 2 405 None 5i07A00 , 2ashA00 , d1iq8b1 PDB mmCIF
57.06 99.26 403 2 404 None 3bldA00 PDB mmCIF
82.15 99.51 404 2 405 None 4gd0A00 , 2ashA00 , d1iq8b1 PDB mmCIF
78.43 99.51 404 2 405 None 3bldA00 , 2ashA00 , d1iq8b1 PDB mmCIF
Model Quality Coverage Length Model Start Model End Ligand Templates Download
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